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基于SSR标记的8个山荆子居群遗传多样性和遗传关系分析
引用本文:王雷宏,郑玉红,汤庚国.基于SSR标记的8个山荆子居群遗传多样性和遗传关系分析[J].植物资源与环境学报,2012,21(1):42-46.
作者姓名:王雷宏  郑玉红  汤庚国
作者单位:1. 安徽农业大学林学与园林学院,安徽合肥,230036
2. 江苏省·中国科学院植物研究所(南京中山植物园),江苏南京210014
3. 南京林业大学森林资源与环境学院,江苏南京,210037
基金项目:安徽省自然科学基金资助项目(10040606Q18)
摘    要:采用10对SSR引物对8个山荆子Malus baccata (L.) Borkh.]居群140个单株的基因组总DNA进行PCR扩增,并据此对8个居群的遗传多样性和遗传关系进行了分析.结果表明:用10对SSR引物共扩增出91条带,多态性条带百分率达100.00%.8个居群的遗传多样性参数差异较大,有效等位基因数为1.437 9~1.535 0,Nei's基因多样性指数为0.256 0~0.309 2,Shannon信息指数为0.376 7~0.459 2,多态性条带百分率为64.84%~85.71%.居群间的有效等位基因数为1.616 9,Nei's基因多样性指数为0.355 1,Shannon信息指数为0.528 5,均明显高于居群内;8个居群间的基因流为1.739 5,基因分化系数为0.223 3,显示居群间的基因交换较多.UPGMA聚类分析结果表明:在Nei's遗传距离0.148 6处,8个居群被分为3组,河北塞罕坝居群单独为一组,山西五台山居群和北京东灵山居群为一组,其余5个居群为一组.据此推测:山荆子起源于中国华北和东北地区,山西灵空山、黑龙江小兴安岭、吉林长白山和山西中条山居群可能是其遗传多样性的核心居群.

关 键 词:山荆子  居群  遗传多样性  遗传关系  SSR标记  聚类分析

Analyses of genetic diversity and genetic relationship of eight populations of Malus baccata based on SSR marker
WANG Lei-hong , ZHENG Yu-hong , TANG Geng-guo.Analyses of genetic diversity and genetic relationship of eight populations of Malus baccata based on SSR marker[J].Journal of Plant Resources and Environment,2012,21(1):42-46.
Authors:WANG Lei-hong  ZHENG Yu-hong  TANG Geng-guo
Institution:1.School of Forestry and Landscape of Architecture,Anhui Agricultural University,Hefei 230036,China;2.Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences,Nanjing 210014,China;3.College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037,China)
Abstract:PCR amplification of total genomic DNA from 140 individuals of eight populations of Malus baccata(L.) Borkh.was carried out by using ten pairs of SSR primers,and hereby,their genetic diversity and genetic relationship were analyzed.The results show that there are totally 91 bands amplified by ten pairs of SSR primers and percentage of polymorphic band is 100.00%.The difference of genetic diversity parameters of eight populations is great,and effective number of allele is 1.437 9-1.535 0,Nei’s gene diversity index is 0.256 0-0.309 2,Shannon information index is 0.376 7-0.459 2,percentage of polymorphic band is 64.84%-85.71%.The effective number of allele,Nei’s gene diversity index and Shannon information index among eight populations are 1.616 9,0.355 1 and 0.528 5,respectively,which is obviously higher than those within population.The gene flow and gene differentiation coefficient of eight populations are 1.739 5 and 0.223 3,respectively,indicating that the gene exchange among populations is more.The result of UPGMA cluster analysis shows that eight populations of M.baccata are divided into three groups where Nei’s genetic distance is 0.148 6.In which,the population in Saihanba of Hebei Province is a group individually,two populations in Mt.Wutai of Shanxi Province and Mt.Dongling of Beijing City are a group,the other five populations are a group.Based on these results,it is presumed that M.baccata is originated from the North and Northeast of China,M.baccata populations in Mt.Lingkong of Shanxi Province,Xiaoxing’anling of Heilongjiang Province,Mt.Changbai of Jilin Province and Mt.Zhongtiao of Shanxi Province may be core populations of genetic diversity.
Keywords:Malus baccata(L  ) Borkh    population  genetic diversity  genetic relationship  SSR marker  cluster analysis
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