不同栽培龄期光果甘草不同部位总黄酮含量的分析

光果甘草(Glycyrrhiza glabra L.)为甘草属(Glycyrrhiza L.)多年生草本植物,是药用甘草的原植物之一1].甘草属植物所含的黄酮类成分具有抗血栓、抗氧化、抗肿瘤、抗衰老、增加白细胞、抗动脉硬化、抗心律失常和抑制HIV等作用2-5];光果甘草中的黄酮类成分光甘草定具有良好的抗氧化、抗炎及抗菌作用,应用前景广阔6].目前关于光果甘草总黄酮含量已有一些报道7-8],但有关生长年限对栽培光果甘草总黄酮含量的影响却很少报道9-10],尤其是对不同栽培龄期光果甘草在不同采集时间各部位总黄酮含量的变化规律缺乏较全面的研究,致使光果甘草药材生产缺乏有力的理论指导依据.

Analysis of total flavonoids content in different parts of Glycyrrhiza glabra with different cultivated ages

The total flavonoids content in different parts(leaf,stem,root and rhizome) of two-year-old and eight-year-old cultivated Glycyrrhiza glabra L.collected in April,June,August and October were determined by UV-visible spectrophotometry method.The result shows that the average content of total flavonoids of two-year-old and eight-year-old plants reaches the highest in June with the average content of 35.55 and 39.97 mg·g-1,respectively.According to the average content of total flavonids in different parts of two ages plants,the order from the highest to the lowest is basically same,all appear that upper leaf is the highest,middle leaf is the second,different under-ground parts are middle,and lower stem is the lowest.The comprehensive analysis results indicate that there are no obvious differences in change regularity of total flavonoids content in G.glabra with different cultivated ages.Two-year-old cultivated G.glabra could be harvested,the optimal harvesting time is October,and the harvesting parts are leaf and under-ground part.