| 红凤菜新鲜茎叶中总黄酮提取物的LC-MS分析 |
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| 引用本文： | 任冰如,吕寒,陈剑,梁呈元,吴菊兰,李维林.红凤菜新鲜茎叶中总黄酮提取物的LC-MS分析[J].植物资源与环境学报,2014(3):108-110. |
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| 作者姓名： | 任冰如 吕寒 陈剑 梁呈元 吴菊兰 李维林 |
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| 作者单位： | 江苏省·中国科学院植物研究所 南京中山植物园 江苏省药用植物研究开发中心 江苏省抗糖尿病药物筛选技术服务中心，江苏 南京 210014 |
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| 基金项目： | 江苏省产学研联合创新资金项目，江苏省科技基础设施建设计划---科技公共服务平台项目 |
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| 摘 要： | 红凤菜（Gynura bicolor DC.）为菊科（Compositae）菊三七属（Gynura Cass.）植物，别名血皮菜、观音菜、观音苋、紫背天葵等，主要分布于中国南方各地，全草均可入药1]；也可作为蔬菜食用，在国内多个地区均有栽培和销售。目前已知红凤菜含有黄酮类、酚性酸类、萜类、甾醇类、脂肪酸类、生物碱类及花青素类等2-7]成分。由于黄酮类成分多具有保护心血管、抗肿瘤、抗糖尿病、抗氧化、抗炎和抗病毒等作用8]，因此对红凤菜中黄酮类成分的研究具有深度开发价值。
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| 关 键 词： | 红凤菜 LC—MS 总黄酮 槲皮素 山柰酚 |
LC-MS analysis of total flavonoids extracts from fresh stem and leaf of Gynura bicolor |
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| REN Bingru,LYU Han,CHEN Jian,LIANG Chengyuan,WU Julan,LI Weilin.LC-MS analysis of total flavonoids extracts from fresh stem and leaf of Gynura bicolor[J].Journal of Plant Resources and Environment,2014(3):108-110. |
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| Authors: | REN Bingru LYU Han CHEN Jian LIANG Chengyuan WU Julan LI Weilin |
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| Institution: | REN Bingru;LYU Han;CHEN Jian;LIANG Chengyuan;WU Julan;LI Weilin;Jiangsu Center for Research and Development of Medicinal Plants,Jiangsu Provincial Service Center for Anti-diabetic Drugs Screening,Institute of Botany,Jiangsu Province and the Chinese Academy of Sciences; |
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| Abstract: | Four total flavonoids extracts were obtained from fresh stem and leaf of Gynura bicolor DC. by method of ethanol extraction-macroporous resin column chromatography-polyamide column chromatography-recrystallization, and their compositions were analyzed by LC-MS technology. The results show that there are six groups of flavonoid absorption peak in total flavonoids extracts of G. bicolor, which is identified to be eight components including rutin, quercetin-3-O- rubinobioside, hyperoside, isoquercitrin, kaempferol-3-O-rubinobioside, kaempferol-3-O-rutinoside, kaempferol-3-O- galactoside and astragalin. This extraction method can effectively extract and purify total flavonoids from fresh stem and leaf of G. bicolor, so it can be used for factory production. |
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| Keywords: | Gynura bicolor DC LC-MS total flavonoids quercetin kaempferol |
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