箭叶秋葵愈伤组织诱导及增殖培养的激素组合筛选

箭叶秋葵 Abelmoschus sagittifolius(Kurz.) Merr.]又名红花马宁、铜皮、五指山参、小红芙蓉、岩酸,系锦葵科(Malvaceae)秋葵属(Abelmoschus Medic.)多年生亚灌木状草本;其根既可食用又可入药,具有滋养强壮之功效,可用于治疗神经衰弱、头晕、腰腿痛、胃痛及腹泻等1].

Selection of hormone combination for induction and proliferation cultures of callus from Abelmoschus sagittifolius

Taking cotyledon and hypocotyl of Abelmoschus sagittifolius(Kurz.) Merr.as explants,the optimal hormone combinations for induction and proliferation cultures of their calli were selected.The results show that the growth status of callus is better with an induction rate of 100.0% on MS medium(containing 5.5 g·L-1 agar and 30 g·L-1 sugar,pH 6.0) with 1.0 mg·L-1 2,4-D and 0.1 mg·L-1 KT,so this hormone combination is suitable for callus induction.And cotyledon is better than hypocotyl as explant for callus induction of the species.The effect of callus proliferation is the best with an average increment of 9.74 g per culture bottle on MS medium with 2 mg·L-1 6-BA and 1.0 mg·L-1 NAA,so this hormone combination is optimal for callus proliferation culture of A.sagittifolius cotyledon.