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杠柳根皮乙醇提取液对蔬菜害虫小菜蛾的生物活性
引用本文:朱九生,乔雄梧,王静,秦曙.杠柳根皮乙醇提取液对蔬菜害虫小菜蛾的生物活性[J].植物资源与环境学报,2004,13(3):31-34.
作者姓名:朱九生  乔雄梧  王静  秦曙
作者单位:山西省农药重点实验室,山西省农业科学院植物保护研究所,山西,太原,030031
基金项目:山西省科技攻关项目,山西省青年基金
摘    要:采用95%乙醇对杠柳(Periploca sepium Bunge)根皮进行热提取,以叶片浸渍法和点滴法测定了提取液对小菜蛾(Plutella xylostella L.)的杀虫活性及其作用方式.结果显示,杠柳乙醇提取液稀释100倍处理对小菜蛾3龄和4龄幼虫24 h后的非选择性拒食率分别为87.3%和96.3%;100倍液浸叶饲喂处理对小菜蛾2龄幼虫72 h后的校正死亡率为80%,对小菜蛾3龄幼虫24 h和48 h后的生长抑制率为100%.杠柳乙醇提取液对小菜蛾幼虫具有较高的生物活性,其作用方式包括拒食作用、胃毒作用和生长抑制作用.此外,乙醇提取液对小菜蛾幼虫还有一定的触杀和内吸效应,并对小菜蛾成虫产卵有明显的忌避活性,但对小菜蛾卵没有杀伤作用.

关 键 词:杠柳  乙醇提取液  生物活性  小菜蛾
文章编号:1004-0978(2004)03-0031-04
修稿时间:2004年3月12日

The bioactivity of the ethanol extracts from root bark of Periploca sepium against Plutella xylostella
ZHU Jiu-sheng.The bioactivity of the ethanol extracts from root bark of Periploca sepium against Plutella xylostella[J].Journal of Plant Resources and Environment,2004,13(3):31-34.
Authors:ZHU Jiu-sheng
Institution:ZHU Jiu-sheng~
Abstract:The root barks of Periploca sepium Bunge were hot-extracted with 95% ethanol, and leaf immersion method and topical application were used to test the bioactivity of the extracts against Plutella xylostella L. The results indicated that antifeeding rate was 87.3% and 96.3% against 3rd and 4th instar larvae of P. xylostella after 24 h of treatment with 100 times dilution of the ethanol extracts in no-choice test respectively. The corrected mortality of the larvae within 72 h reached 80% when the 2nd instar larvae of P. xylostella were fed by the leaf discs treated with 100 times dilution of the extracts. The growth inhibition rate of 100 times dilution of the extract was 100% to 3rd instar larvae. It is clear that the ethanol extracts has strong antifeeding property, stomach poison and growth inhibition to larvae of P. xylostella. In addition, the ethanol extracts of P. sepium possesses certain contact poison and systemic toxicity on the insect. The experimental results also showed that the crude extracts had obvious deterrent effect against the oviposition of P. xylostella. However, the extracts showed no killing effect on the eggs of P. xylostella.
Keywords:Periploca sepium Bunge  ethanol extracts  bioactivity  Plutella xylostella L  
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