土壤干旱胁迫对酸枣叶片黄酮类代谢及某些生长和生理指标的影响

采用盆栽称量法研究了对照、中度和重度干旱(土壤相对含水量分别为75%、55%和35%)条件下1年生酸枣〔Ziziphus jujuba Mill.var.spinosa(Bunge)Hu ex H.F.Chow〕幼苗叶片中黄酮类成分含量及一些生长和生理指标的变化,并讨论了酸枣适应干旱过程中黄酮类成分的作用。结果表明:在中度和重度干旱条件下酸枣叶片苯丙氨酸解氨酶(PAL)活性均高于对照,但经重度干旱处理后复水第7天PAL活性与对照无显著差异。与对照相比,中度干旱条件下叶片槲皮素含量基本无变化,总黄酮和芦丁含量分别显著或极显著增加,丙二醛和可溶性蛋白质含量无显著变化;与中度干旱条件相比,重度干旱条件下叶片总黄酮、芦丁和槲皮素含量显著或极显著下降,丙二醛含量不显著增加,可溶性蛋白质含量显著降低;与对照相比,重度干旱条件下总黄酮含量无明显变化,芦丁和丙二醛含量极显著或显著增加,槲皮素和可溶性蛋白质含量显著下降;复水第7天,总黄酮、芦丁和槲皮素含量均显著或极显著高于复水前,且总黄酮和芦丁含量显著高于对照,槲皮素含量与对照无显著差异;复水第1天至第4天,丙二醛含量呈先降后增再降的趋势、可溶性蛋白质含量则呈先降低后逐渐增加的趋势,其中复水第1天丙二醛含量显著高于复水前、可溶性蛋白质含量显著低于复水前。在中度或重度干旱胁迫后酸枣枝条长度均极显著小于对照,且随土壤相对含水量的降低枝条长度减小;叶片相对含水量也表现出随土壤相对含水量的降低逐渐减小的趋势,但差异不显著。研究结果提示:适宜的干旱胁迫可促进酸枣叶片黄酮类代谢,但在不同的干旱胁迫条件下,黄酮类代谢在酸枣抗旱过程中具有不同的作用。

Effects of drought stress in soil on flavonoids metabolism in leaf and some growth and physiological indexes of Ziziphus jujuba var.spinosa

Changes of flavonoids component content in leaf and some growth and physiological indexes of one-year-old seedlings of Ziziphus jujuba Mill.var.spinosa(Bunge) Hu ex H.F.Chow under control,moderate drought and heavy drought conditions(relative water content in soil with 75%,55% and 35%,respectively) were studied by pot weighing method,and role of flavonoids component in adapting process to drought was discussed.The results show that PAL activity in Z.jujuba var.spinosa leaf under moderate and heavy drought c...