Production of a soluble and secreted antigenic fragment of HBsAg in yeast. |
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Authors: | E H Baba I Berkower |
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Institution: | Departamento de Bioquimica-ICB-UFMG, ICEB-Universidade Federal de Ouro Preto, Brazil. |
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Abstract: | We have produced a fragment of hepatitis B surface antigen (HBsAg) corresponding to amino acids 1-60 as a fusion protein with the alpha mating factor of yeast. The product is secreted from yeast as a soluble monomer that expresses HBsAg antigenicity. Unlike other heterologous fusion proteins, it is not processed by the Lys-Arg endoprotease, possibly due to a proline in the linker between the two coding sequences. The resulting soluble fragment will enable us to map the immunodominant sites of HBsAg recognized by T cells and to identify additional factors contributing to vaccine potency. |
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