Biological activity of the antitumor protein neocarzinostatin coupled to a monoclonal antibody by N-succinimidyl 3-(2-pyridyldithio)-propionate期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Biological activity of the antitumor protein neocarzinostatin coupled to a monoclonal antibody by N-succinimidyl 3-(2-pyridyldithio)-propionate

Authors:	Gundram Jung Wolfgang Köhnlein Gerd Lüders

Institution:	Institut für Strahlenbiologie der Westfälischen Wilhelms Universität, 4400 Münster, Germany

Abstract:	The chromophore free apoprotein of neocarzinostatin was coupled to monoclonal IgG₁ antibody using N-Succinimidyl 3-(2-pyridyldithio)-propionate as heterobifunctional reagent. After coupling active chromophore was reassociated with the apoprotein. We present here experimental evidence that the hybrid protein retains biological activity as measured by the degradation of T2-DNA and bacteriostatic action.

Keywords:	NCS neocarzinostatin apo-NCS chromophore free neocarzinostatin SPDP N-succinimidyl 3-(2-pyridyldithio)-propionate DTT dithiothreitol PBS phosphate buffered saline ABS acetate buffered saline
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