Negatively supercoiled DNA from plants infected with a single-stranded DNA virus |
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| Authors: | G Sunter R H Coutts K W Buck |
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| Institution: | Department of Pure and Applied Biology, Imperial College of Science and Technology, London SW7 2BB, UK |
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| Abstract: | A method for isolating covalently closed circular double-stranded DNA from plants infected with the geminivirus, tomato golden mosaic virus, is described. Ethidium bromide titration showed this DNA to be negatively supercoiled with a superhelical density of -0.062. The presence of S1 nuclease-sensitive secondary structure in the supercoiled DNA was demonstrated by its conversion to the open circular and linear DNA forms on treatment with this enzyme. |
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| Keywords: | TGMV tomato golden mosaic virus RF replicative form |
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