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Nucleotide sequence of the genes for F0 components of the proton-translocating ATPase from Escherichia coli: prediction of the primary structure of F0 subunits
Authors:H Kanazawa  K Mabuchi  T Kayano  T Noumi  T Sekiya  M Futai
Institution:1. Department of Microbiology, Faculty of Pharmaceutical Sciences Okayama University, Okayama 700, Japan;2. Department of Biology, National Cancer Center Research Institute Tokyo 104, Japan
Abstract:The 1763 nucleotide-long-DNA sequence of part of the gene cluster for the proton-translocating ATPase from E. coli was determined. The sequence covers the genes for the a and b subunits of F0 along with the intercistronic regions. In the region preceding the gene for the a subunit, a reading frame encompassing 127 amino acids was found. The primary structure of the a and b subunits were deduced and the properties of these proteins were predicted. Analysis of codon usage in these genes was made.
Keywords:tRNA  transfer RNA  peripheral and integral portions of proton-translocating ATPase  a  b  and c subunits
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