Essentiality of insulin and prolactin for accumulation of rat casein mRNAs |
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| Authors: | J K Kulski K R Nicholas Y J Topper P Qasba |
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| Institution: | 1. Department of Neuroscience, Johns Hopkins University School of Medicine 725 North Wolfe Street Baltimore, Maryland 21205 USA;2. Department of Pharmacology and Experimental Therapeutics, Johns Hopkins University School of Medicine 725 North Wolfe Street Baltimore, Maryland 21205 USA;3. Department of Psychiatry and Behavioral Sciences Johns Hopkins University School of Medicine 725 North Wolfe Street Baltimore, Maryland 21205 USA;4. Center for Laboratories and Research, New York State Department of Health Albany, New York 12201 USA |
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| Abstract: | Fetal calf serum (20%) does not substitute for insulin in terms of enhanced accumulation of rat casein mRNAs, but it can sustain the mammary tissue in culture. This property of fetal calf serum was utilized to show that in the presence of cortisol and 1) insulin or 2) prolactin, the delayed addition of prolactin or insulin, respectively, produced similar enhancement of 25 K and 42 K casein mRNAs. The results indicate that both insulin and prolactin are required for accumulation of rat casein mRNAs, the accumulation is independent of the sequence of addition of these hormones, and that the response cannot be ascribed entirely to the hormone added last. |
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| Keywords: | GPSA guanidinopropylsuccinic acid GEMSA guanidinoethylmercaptosuccinic acid APMSA aminopropylmercaptosuccinic acid MGTA 2-mercaptomethyl-3-guanidinoethylthiopropanoic acid |
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