Molecular characterization of a stable Flac plasmid |
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Authors: | F L Macrina E Balbinder A Bassel |
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Institution: | Department of Biology, Syracuse University Syracuse, NY 13210 USA;Department of Microbiology State University of New York Upstate Medical Center Syracuse, NY 13210 USA |
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Abstract: | FS is a thermostable extrachromosomal element isolated in which is altered in its replication as compared to its precursor Fts114. Sedimentation of both these plasmids in alkaline sucrose gradients has indicated a difference in their sizes. Contour length measurements of open circular plasmid DNA molecules photographed in the electron microscope have revealed the estimated molecular weight of Fts114 to be 81 × 106 daltons while that of FS is 109 × 106 daltons. FS may carry a segment of chromosomal or cryptic plasmid DNA. |
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Keywords: | to whom reprint requests should be addressed |
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