Identification and characterization of a cytosolic 30 kDa histamine stimulated phosphoprotein in parietal cell cytosol |
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| Authors: | M Oddsdottir J R Goldenring T E Adrian M J Zdon K A Zucker I M Modlin |
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| Institution: | Department of Surgery, Yale University School of Medicine, West Haven, Connecticut. |
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| Abstract: | Histamine stimulated acid secretion is mediated by an increase in intracellular cAMP. Cytosolic protein phosphorylation stimulated by histamine was investigated in isolated rabbit parietal cells. Histamine stimulated the phosphorylation of a 30 kDa phosphoprotein with an isoelectric point of 5.6. Cimetidine completely inhibited histamine-stimulated pp30 phosphorylation. However, omeprazole had no effect on the phosphorylation of pp30. Forskolin and 8-bromo-cAMP also stimulated the phosphorylation of pp30. The results suggest that pp30 is a histamine-stimulated, cAMP-dependently phosphorylated protein substrate in parietal cell cytosol. |
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