Purification of the murine heat-stable antigen from erythrocytes. |
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| Authors: | Y Hitsumoto A Nakano H Ohnishi F Hamada S Saheki N Takeuchi |
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| Institution: | Department of Clinical Laboratory Medicine, Ehime University School of Medicine, Japan. |
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| Abstract: | The rat anti-mouse erythrocyte (MRBC) monoclonal antibody (mAb), R13, has been developed. The MRBC membrane protein recognized by R13 (R13-Ag) can be purified by loading the butanol-extracted MRBC membrane solution on a R13-conjugated Cellulofine column in the presence of 0.1% CHAPS followed by elution with 1% CHAPS. The amino acid sequence of the affinity-purified R13-Ag corresponded to that predicted from the cDNA for the murine heat-stable antigen. It was revealed that the actual heat-stable antigen was composed of 27 amino acids. |
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