Effects of thiamine and pyridoxine on the content and composition of sterols in <span class="math"><math><mtext>Saccharomyces carlsbergensis</mtext></math></span> 4228期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

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Effects of thiamine and pyridoxine on the content and composition of sterols in $Saccharomyces carlsbergensis$ 4228

Authors:	Jun Nagai Hirohiko Katsuki Yoshiki Nishikawa Ichiro Nakamura Teijiro Kamihara Saburo Fukui

Institution:	Department of Chemistry, Faculty of Science, Kyoto University, Kyoto, Japan;Laboratory of Industrial Biochemistry, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Kyoto, Japan

Abstract:	The level of sterols in $S. carlsbergensis$ 4228 cells grown aerobically on a synthetic medium fortified with thiamine was significantly low compared with that in the control cells. The levels of free and esterified sterols in the thiamine-cells were 60% and 10% of the corresponding sterol levels in the control cells, respectively. Analysis by gas-liquid chromatography of non-saponifiable lipids extracted from the cells revealed that the amounts of squalene, lanosterol and two unidentified sterols were higher than those in the control cells and that ergosterol and zymosterol, major sterols in the control cells, were not present. These effects of thiamine on the content and composition of sterols were abolished by the addition of pyridoxine to the medium.

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