Cycloastragenol,a triterpene aglycone derived from Radix astragali, suppresses the accumulation of cytoplasmic lipid droplet in 3T3-L1 adipocytes |
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Authors: | Shifeng Wang Chenxi Zhai Qing Liu Xing Wang Zhenzhen Ren Yuxin Zhang Yanling Zhang Qinghua Wu Shengnan Sun Shiyou Li Yanjiang Qiao |
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Institution: | 1. School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing, China;2. HD Biosciences Co., Ltd., Shanghai, China;3. Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing, China |
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Abstract: | Cycloastragenol (CAG), a bioactive triterpenoid sapogenin isolated from the Chinese herbal medicine Radix astragali, was reported to promote the phosphorylation of extracellular signal-regulated protein kinase (ERK). Here we investigated the effect of CAG on adipogenesis. The image-based Nile red staining analyses revealed that CAG dose dependently reduced cytoplasmic lipid droplet in 3T3-L1 adipocytes with the IC50 value of 13.0 μM. Meanwhile, cytotoxicity assay provided evidence that CAG was free of injury on HepG2 cells up to 60 μM. In addition, using calcium mobilization assay, we observed that CAG stimulated calcium influx in 3T3-L1 preadipocytes with a dose dependent trend, the EC50 value was determined as 21.9 μM. There were proofs that elevated intracellular calcium played a vital role in suppressing adipocyte differentiation. The current findings demonstrated that CAG was a potential therapeutic candidate for alleviating obesity and hyperlipidemia. |
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Keywords: | RA Radix astragali CAG cycloastragenol AG-IV astragaloside IV ERK extracellular signal-regulated protein kinase PFA paraformaldehyde Lova Lovastatin GSH reduced glutathione level ROS reactive oxygen species MMP mitochondrial membrane potential [Ca2+]i intracellular calcium 2APB 2-aminoethoxydiphenyl borate IBMX 3-isobutyl-1-methylxanthine TRP transient receptor potential TRPV1 transient receptor potential V1 TRPV2 transient receptor potential V2 TRPA1 transient receptor potential Ankyrin 1 IC50 50% inhibitory concentration |
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