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Systemic identification of estrogen-regulated genes in breast cancer cells through cap analysis of gene expression mapping |
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| Authors: | Ryonosuke Yamaga Kazuhiro Ikeda Joost Boele Kuniko Horie-Inoue Ken-ichi Takayama Tomohiko Urano Kaoru Kaida Piero Carninci Jun Kawai Yoshihide Hayashizaki Yasuyoshi Ouchi Michiel de Hoon Satoshi Inoue |
| Institution: | 1. Division of Gene Regulation and Signal Transduction, Research Center for Genomic Medicine, Saitama Medical University, Saitama, Japan;2. Department of Geriatric Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;3. Department of Anti-Aging Medicine, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan;4. Department of Systems Bioinformatics, Amsterdam Institute for Molecules, Medicines and Systems, VU University Amsterdam, Amsterdam, the Netherlands;5. RIKEN Center for Life Science Technologies, Division of Genomic Technologies, Yokohama, Kanagawa, Japan;6. RIKEN Preventive Medicine and Diagnosis Innovation Program, Wako, Saitama, Japan;g RIKEN Omics Science Center, Yokohama, Kanagawa, Japan1 |
| Abstract: | To explore the estrogen-regulated genes genome-widely in breast cancer, cap analysis of gene expression (CAGE) sequencing was performed in MCF-7 cells under estrogen treatment. Estrogen-regulated expressional changes were found in 1537 CAGE tag clusters (TCs) (?1.5 or ?0.66-folds). Among them, 15 TCs were situated in the vicinity of (?10 kb) reported estrogen receptor-binding sites. Knockdown experiments of the 15 TC-associated genes demonstrated that the genes such as RAMP3, ISOC1 and GPRC5C potentially regulate the growth or migration of MCF-7 cells. These results suggest that CAGE sequencing will reveal novel estrogen target genes in breast cancer. |
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