|
|||||
|
|
Screening of recombinant Escherichia coli using activation of green fluorescent protein as an indicator |
|
| Authors: | Shirou Tsuchida Mao TamuraNaoya Hamaue Takashi Aoki |
| Institution: | Department of Molecular Biosciences, School of Pharmaceutical Sciences, Health Sciences University of Hokkaido, Ishikari-Tobetsu, Hokkaido 061-0293, Japan |
| Abstract: | A novel cloning vector that can be used to identify recombinant Escherichia coli colonies by activation of the green fluorescent protein gene (GFP) was constructed. Screening using the vector does not require special reagents. The recombinant plasmid activates GFP, and the rate of false-positive results is low. |
| Keywords: | E coli Escherichia coli GFP green fluorescent protein IPTG isopropyl-β-d-thiogalactopyranoside LB Luria&ndash Bertani PCR polymerase chain reaction X-gal 5-bromo-4-chloro-3-indolyl-β-d-galactoside |
| 本文献已被 ScienceDirect 等数据库收录！ | |