Addition of mannose to both the amino- and carboxy-terminal propeptides of type II procollagen occurs without formation of a triple helix |
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Authors: | Norberto A Guzman Peter N Graves Darwin J Prockop |
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Institution: | Department of Biochemistry, College of Medicine and Dentistry of New Jersey-Rutgers Medical School, Piscataway, New Jersey 08854 USA |
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Abstract: | Matrix-free cells obtained from chick embryo cartilage were incubated in the presence of α,α′-dipyridyl and radioactive mannose in order to examine the incorporation of mannose into the propeptide extensions of Type II procollagen. Cell proteins were digested with bacterial collagenase and the digests were examined by polyacrylamide gel electrophoresis. Radioactive mannose was found in fragments from both the N- and C-propeptides, and therefore the results provided the first indication that both these propeptides of Type II procollagen contain mannose. The results also supported previous indications that addition of carbohydrate to the propeptides of procollagen does not require folding of the collagen domain into a triple helix. |
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