Tyrosine protein kinase activity in the DMBA-induced rat mammary tumor: inhibition by quercetin |
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| Authors: | J Levy I Teuerstein M Marbach S Radian Y Sharoni |
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| Institution: | Endocrine Laboratory and Department of Clinical Biochemistry, Soroka University Hospital and Faculty of Health Sociences, Ben-Gurion University of the Negev, Beer-Sheva, Israel |
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| Abstract: | Tyrosine protein kinase activity was measured in membranes from DMBA-induced mammary tumors, with Angiotensin II as substrate. The apparent Km for the peptide was 3.3 mM. This enzymatic activity is inhibited by Ca+2; Mn+2 can replace Mg+2 with an increase in the Km for ATP from 47 /microM to 172 microM. The enzymatic activity was not affected by cyclic AMP but was inhibited in dose dependent manner by quercetin, a bioflavonoid which is known to inhibit proliferation of malignant cells in vitro. |
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| Keywords: | Author to whom correspondence should be sent: Dr Yoav Sharoni Endocrinology Laboratory Soroka Medical Center P O Box 151 Beer-Sheva 84101 Israel |
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