Stratum intermedium lineage diverges from ameloblast lineage via Notch signaling |
| |
Authors: | Harada Hidemitsu Ichimori Yasuo Yokohama-Tamaki Tamaki Ohshima Hayato Kawano Shintaro Katsube Ken-ichi Wakisaka Satoshi |
| |
Institution: | Department of Oral Anatomy and Developmental Biology, Osaka University Graduate School of Dentistry, 1-8, Yamadaoka, Suita, Osaka 565-0871, Japan. hide-h@dent.osaka-u.ac.jp |
| |
Abstract: | The stratum intermedium develops as flattened cell layers on the proximal side of the ameloblast layer during tooth development. However, little information is available regarding the origin and the role. In this study, we indicate that some stratum intermedium cells originate from the inner enamel epithelium (IEE) in rat incisor organ cultures using DiI as a tracer. Immunohistochemical and in situ hybridization studies showed that the stratum intermedium cells express the Notch1 protein and Hes1 mRNAs, while the IEE and ameloblasts express the Jagged1. Further, we examined the role of Notch signaling using the dental epithelial cell line HAT-7. Recombinant Jagged1 protein enhanced the appearance of stratum intermedium cells in HAT-7 cultures and neutralization with an anti-Jagged1 antibody inhibited these effects. Additionally, overexpression of the Notch1 internal domain increased the number of stratum intermedium cells. We hypothesize that the stratum intermedium lineage differentiates from the ameloblast lineage via Notch signaling. |
| |
Keywords: | Stratum intermedium Ameloblasts Inner enamel epithelium Notch Jagged DiI Dental epithelial progenitor cells |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|