RPAP3 splicing variant isoform 1 interacts with PIH1D1 to compose R2TP complex for cell survival |
| |
| Authors: | Miki Yoshida Makio Saeki Hiroshi Egusa Yasuyuki Irie Yuya Kamano Shinya Uraguchi Maki Sotozono Hitoshi Niwa Yoshinori Kamisaki |
| |
| Institution: | 1. Douglas Stephens Surgical Research Laboratory, Murdoch Children’s Research Institute, Melbourne, Australia;2. Urology Department, Royal Children''s Hospital, Melbourne, Australia;3. Department of Paediatrics, University of Melbourne, Australia;4. Division of Developmental Biology, Cincinnati Children''s Hospital Research Foundation |
| |
| Abstract: | We previously characterized RNA polymerase II-associated protein 3 (RPAP3) as a cell death enhancer. Here we report the identification and characterization of splicing isoform of RPAP3, isoform 1 and 2. We investigated the interaction between RPAP3 and PIH1 domain containing protein 1 (PIH1D1), and found that RPAP3 isoform 1, but not isoform 2, interacted with PIH1D1. Furthermore, knockdown of RPAP3 isoform 1 by small interfering RNA down-regulated PIH1D1 protein level without affecting PIH1D1 mRNA. RPAP3 isoform 2 potentiated doxorubicin-induced cell death in human breast cancer T-47 cells although isoform 1 showed no effect. These results suggest that R2TP complex is composed of RPAP3 isoform 1 for its stabilization, and that RPAP3 isoform 2 may have a dominant negative effect on the survival potency of R2TP complex. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
