Distinct actions of strontium on mineral formation in matrix vesicles |
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Authors: | Bechkoff Géraldine Radisson Jacqueline Bessueille Laurence Bouchekioua-Bouzaghou Katia Buchet René |
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Institution: | Université de Lyon, Villeurbanne F-69622, France Université Lyon 1, Villeurbanne F-69622, France INSA-Lyon, Villeurbanne F-69621, France CPE Lyon, Villeurbanne F-69616, France CNRS UMR 5246 ICBMS, Villeurbanne F-69622, France |
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Abstract: | Matrix vesicles (MVs) are involved in the initial step of mineralization in skeletal tissues and provide an easily model to analyze the hydroxyapatite (HA) formation. Sr stimulates bone formation and its effect was tested on MVs. Sr2+ (15-50 μM) in the mineralization medium containing MVs, 2 mM Ca2+ and 3.42 mM Pi, retarded HA formation. Sr2+ (1-5 mM) in the same medium-induced other types of mineral than HA and cancelled the ATP-, ADP- or PPi-induced retardation in the mineral formation. Our findings suggest that the beneficial effect of Sr2+ at a low dose (15-50 μM) is rather an inhibitor of bone resorption than an activator of mineral formation, while at high Sr2+ concentration (1-5 mM), mineral formation, especially other types of mineral than HA, is favored. |
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Keywords: | BCIP bromo-chloro-indolyl phosphate HA hydroxyapatite MV matrix vesicle NBT nitroblue tetrazolium PAGE polyacrylamide gel electrophoresis pMV pellet of PI-PLC treated MV PI-PLC phosphatidylinositol phospholipase C PDE phosphodiesterase PME phosphomonoesterase SCL synthetic cartilage lymph sMV supernatant of PI-PLC treated MV TNAP tissue non-specific alkaline phosphatase |
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