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Methylations of histone H3 lysine 9 and lysine 36 are functionally linked to DNA replication checkpoint control in fission yeast |
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| Authors: | Kim Hyun Soo Rhee Dong Keun Jang Yeun Kyu |
| Institution: | a Research Institute, National Cancer Center, Goyang, Gyeonggi 411-769, Republic of Korea b Department of Biology, College of Science, Yonsei University, 134 Shinchon-dong, Seodaemun-gu, Seoul 120-749, Republic of Korea c Yonsei Biomolecule Research Initiative, Yonsei University, Seoul 120-749, Republic of Korea |
| Abstract: | Recently, histone H4 lysine 20 and H3 lysine 79 methylations were functionally linked to DNA damage checkpoint. The crosstalk between histone methylation and the S-M checkpoint, however, has remained unclear. Here, we show that H3 lysine 9 (K9) and lysine 36 (K36) methylations catalyzed by two histone methyltransferases Clr4 and Set2 are involved in hydroxyurea (HU)-induced replication checkpoint. The clr4-set2 double mutants besides histone H3-K9 and K36 double mutants exhibited HU-sensitivity, a defective HU-induced S-M checkpoint, and a significant reduction of HU-induced phosphorylation of Cdc2. Intriguingly, the clr4-set2 double mutations impaired the HU-induced accumulation of a mitotic inhibitor Mik1. Double mutants in Alp13 and Swi6, which can specifically bind to H3-K36 and K9 methylations, exhibited phenotypes similar to those of the clr4-set2 mutants. Together, these findings suggest that methylations of histone H3-K9 and K36 by Clr4 and Set2 are functionally linked to DNA replication checkpoint via accumulation of Mik1. |
| Keywords: | Histone methylation Methylated histone-binding protein Replication checkpoint control Mitotic inhibitor Mik1 Hydroxyurea-induced phosphorylation of Cdc2 Fission yeast |
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