Peroxidase activity in human red cell: a biological model for excited state molecules generation. |
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| Authors: | N Durán Y Makita L H Innocentini |
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| Institution: | Institute de Química, Universidade Estadual de Campinas, C.P. 1170 13100, Campinas, São Paulo, Brazil |
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| Abstract: | The presence of enzymically generated triplet acetone in red cells and energy transfer to eosin, rose bengal and 9,10-dibromoanthracene-2-sulfonate was indicate by: (1) product distribution; (2) KET τo, similar to the 2-methylpropanal/peroxidase/O2 system; (3) correlation between hemolysis, oxygen uptake and photon emission; (4) membrane protection by energy acceptors, and (5) by comparison of the 2-methylpropanal/peroxidase/O2 system with 2-methylpropanal/red cells/membranes/O2 and 2-methylpropanal/acid extractable protein from red cells membrane/O2 systems, which have a high peroxidase activity.This is the first report of a biological system producing a photohemolysis effect in the dark. |
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| Keywords: | To whom inquiries should be addressed |
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