8-nitroguanine formation in the liver of hamsters infected with Opisthorchis viverrini |
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Authors: | Pinlaor Somchai Yongvanit Puangrat Hiraku Yusuke Ma Ning Semba Reiji Oikawa Shinji Murata Mariko Sripa Banchob Sithithaworn Paiboon Kawanishi Shosuke |
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Institution: | Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. |
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Abstract: | Nucleic acid damage by reactive nitrogen and oxygen species may contribute to the carcinogenesis associated with chronic infection and inflammation. We examined 8-nitroguanine and 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) formation and nitric oxide (NO) production in hamsters infected with Opisthorchis viverrini (OV). Formation of 8-nitroguanine was assessed immunohistochemically with an antibody specific for 8-nitroguanine. 8-nitroguanine formation was found mainly in the cytoplasm and slightly in the nucleus of inflammatory cells and epithelial lining of bile duct at inflammatory areas in the liver. 8-nitroguanine immunoreactivity reached the highest intensity on day 30. A time profile of 8-nitroguanine formation was closely associated with that of plasma nitrate/nitrite. HPLC with an electrochemical detector revealed that the amount of 8-oxodG in the liver reached the maximal level on day 21. The mechanisms of 8-oxodG and 8-nitroguanine formation via O2*- and NO production triggered by OV infection were discussed in relation to cholangiocarcinoma development. |
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Keywords: | 8-Nitroguanine 8-oxodG Nitric oxide Reactive oxygen species Eosinophil Macrophage Opisthorchis viverrini Cholangiocarcinoma |
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