Enhancer-site downstream binding protein activity is enriched in rat tissues that express the class I alcohol dehydrogenase gene. |
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Authors: | M F Canto M M Oliva J J Potter E Mezey V W Yang |
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Institution: | Department of Medicine, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205. |
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Abstract: | The activity of the rat class I alcohol dehydrogenase (ADH) is enriched in certain tissues including the liver, intestine and testis. The tissue-specific expression of the gene encoding ADH in the rat was studied and found to closely correlate with tissue isozymic activity. A factor designated enhancer-site downstream binding protein (EDBP) was recently identified in the rat liver and found to interact with the proximal promoter of the class I ADH gene. The distribution of EDBP in nuclear extracts obtained from various tissues was examined based on its sequence-specific DNA binding property and found to correlate with tissue ADH expression. These findings suggest that EDBP is potentially a positive regulatory factor which is involved in controlling the tissue-specific expression of the ADH gene. |
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