|
|||||
|
|
Involvement of microtubule-associated protein 2 (MAP2) in oral cancer cell motility: a novel biological function of MAP2 in non-neuronal cells |
|
| Authors: | Liu Shyun-Yeu Chen Yi-Ting Tseng Mei-Yu Hung Chih-Chang Chiang Wei-Fan Chen Hau-Ren Shieh Tien-Yu Chen Chung-Ho Jou Yuh-Shan Chen Jeff Yi-Fu |
| Institution: | a Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Tainan, Taiwan b Department of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan c Department of Biotechnology, Kaohsiung Medical University, 100, Shih-Chuan 1st Road, San-Ming District, Kaohsiung, Taiwan d Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, Taiwan e Department of Life Science and Institute of Molecular Biology, National Chung Cheng University, Chia-Yi, Taiwan f Department of Oral Maxillofacial Surgery, Kaohsiung Medical University, Kaohsiung, Taiwan g Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan |
| Abstract: | Microtubule-associated protein 2 (MAP2) has been better known for its well-defined role primarily in neurite outgrowth during neuronal development. However, the biological functions of MAP2 in non-neuronal cells, such as epithelial cells, remain largely unknown. In the present study, we sought to investigate the cellular functions of MAP2 by separately establishing stable expression of two MAP2 isoforms, MAP2A and MAP2C, in oral squamous cell carcinoma, Ca9-22. Ectopic expression of MAP2A or MAP2C results in microtubule bundling predominantly at the cell periphery. Remarkably, overexpression of MAP2A but not MAP2C significantly promotes migration of Ca9-22 cells, whereas knockdown of MAP2A expression by specific siRNA oligos dramatically decreases cell migration of HaCaT, an immortalized keratinocyte cell line with abundant endogenous MAP2A. Furthermore, by immunohistochemical studies, MAP2A was shown to highly and selectively express in invasive oral cancer tissues, consistent with its motility-promoting cellular function revealed through in vitro assays. Thus, our findings have not only identified a novel role of MAP2 in non-neuronal cells, but also provided the first implication of MAP2 in malignant oral cancer tissues. |
| Keywords: | Microtubule-associated protein 2 (MAP2) Oral cancer cells Microtubule bundling Cell migration Invasive oral cancer tissues |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ | |