Existence of Met-enkephalin-Arg6-Gly7-Leu8 with Met-enkephalin,Leu-enkephalin and Met-enkephalin-Arg6-Phe7 in the brain of guinea pig,rat and golden hamster |
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| Authors: | Yoshio Ikeda Kazuwa Nakao Takaaki Yoshimasa Noboru Yanaihara Shosaku Numa Hiroo Imura |
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| Institution: | 1. Department of Medicine, Kyoto University School of Medicine, Kyoto 606, Japan.;2. Department of Medical Chemistry (S.N.), Kyoto University School of Medicine, Kyoto 606, Japan.;3. Shizuoka Pharmaceutical College (N.Y.), Shizuoka, Japan. |
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| Abstract: | High performance liquid chromatography (HPLC) coupled with specific radioimmunoassays for methionine-enkephalin-Arg6-Gly7-Leu8 (Met-E-Arg6-Gly7-Leu8), methionine-enkephalin (Met-E), leucine-enkephalin (Leu-E) and methionine-enkephalin-Arg6-Phe7 (Met-E-Arg6-Phe7) has demonstrated that Met-E-Arg6-Gly7-Leu8 exists together with Met-E, Leu-E and Met-E-Arg6-Phe7 in the brain of guinea pig, rat and golden hamster. The content of Met-E-Arg6-Gly7-Leu8 was comparable to those of Leu-E and Met-E-Arg6-Phe7, whereas that of Met-E was the highest among the four opioid peptides. These results are compatible with the recent studies on the nucleotide sequence of cloned cDNA for preproenkephalin from bovine adrenal medulla, which reveal that this precursor molecule contains four copies of Met-E and one copy each of Leu-E, Met-E-Arg6-Phe7 and Met-E-Arg6-Gly7-Leu8. The co-existence of Met-E-Arg6-Gly7-Leu8 with Met-E, Leu-E and Met-E-Arg6-Phe7 suggests that their biosynthetic pathway in the brain is similar to that in the adrenal medulla. |
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| Keywords: | Met-E Tyr-Gly-Gly-Phe-Met Leu-E Tyr-Gly-Gly-Phe-Leu Tyr-Gly-Gly-Phe-Met-Arg-Gly-Leu Tyr-Gly-Gly-Phe-Met-Arg-Phe HPLC high performance liquid chromatography RIA radioimmunoassay ODS octadecylsilane LI like immunoreactivity |
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