Brain CCK receptors are structurally distinct from pancreas CCK receptors |
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Authors: | C Sakamoto J A Williams I D Goldfine |
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Institution: | 1. Cell Biology Laboratory, Mount Zion Hospital and Medical Center, San Francisco, CA 94120 USA;2. Department of Physiology, University of California, San Francisco, CA 94143 USA;3. Department of Medicine, University of California, San Francisco, CA 94143 USA |
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Abstract: | Brain and pancreas cholecystokinin (CCK) receptors differ markedly in their selectivity for CCK analogs. To determine the size and subunit structure of the brain CCK receptor and compare it to that of the pancreas, 125I-CCK33 was covalently cross-linked with ultraviolet light to its receptor on mouse brain particles and purified pancreatic plasma membranes. When CCK was crosslinked to brain membranes, a single consistent major labeled protein band of Mr = 55,000 was observed in both the presence and the absence of DTT. These data with brain receptors contrast to results with pancreatic receptors where two bands of Mr = 120,000 and 80,000 are labeled in the absence and presence of DTT, respectively. These studies indicate, therefore, that the brain and pancreas CCK receptors are structurally and functionally distinct. |
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Keywords: | CCK cholecystokinin carboxyl-terminal octapeptide of CCK carboxyl terminal tetrapeptide of CCK MES 2-(N-morpholino)ethanesulfonic acid HEPES N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid PMSF phenylmethanesulfonyl fluoride EGTA ethylene glycol bis (β-aminoethyl ether) N N N′ N′-tetraacetic acid DTT dithiothreitol |
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