Structural analysis of the intracellular domain of (pro)renin receptor fused to maltose-binding protein |
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Authors: | Zhang Yanfeng Gao Xiaoli Michael Garavito R |
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Institution: | aCollege of Environmental Science and Engineering, Huazhong University of Science and Technology, Wuhan 430074, PR China;bState Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan 430070, PR China;cPhotochemistry Laboratory, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100080, PR China;dHubei Research Institute of Products Quality Supervision and Inspection, Wuhan 430061, PR China |
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Abstract: | In Synechocystis sp. PCC 6803, the loop domain (aa 1–70) of the phycobilisome core-membrane linker, LCM, was found to interact with the glycosyl transferase homolog, Sll1466. Growth of a Sll1466 knock-out mutant was slightly faster in low light, but strongly inhibited in high light; the phenotype is discussed in relation to the regulation of light energy transfer to photosystem II. At the molecular level, the mutant shows the following changes compared to the wild type: (1) a smaller size and higher mobility of phycobilisomes on the thylakoid membrane, and (2) a changed lipid composition of the thylakoid membrane, especially decreased amounts of digalactosyl diacylglycerol. These results indicate a profound regulatory role for Sll1466 in regulating photosynthetic energy transfer. |
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Keywords: | Abbreviations: ApcE core-membrane linker LCM DBMIB 2 5-dibromo-3-methyl-6-isopropyl-p-benzoquinone DCMU 3-(3 4-dichlorophenyl)-1 1-dimethylurea DGDG digalactosyl diacylglycerol ET energy transfer GT glycosyl transferase HL high light KPB potassium phosphate buffer LL low light MGDG monogalactosyl diacylglycerol ML medium light PAM pulse amplitude modulated fluorescence PBS phycobilisome PG phosphatidyl glycerol PS photosystem SQDG sulfoquinovosyl diacylglycerol wt wild type |
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