Use of 9-aminoacridine as a probe in a kinetic study of DNA-poly-L-lysine interaction |
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| Authors: | H Hasumi K Akasaka H Hatano K Hiromi |
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| Institution: | 1. Department of Chemistry, Faculty of Science, Kyoto University, Kyoto 606, Japan;2. Department of Food Science and Technology, Faculty of Agriculture, Kyoto University, Kyoto 606, Japan |
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| Abstract: | An attempt has been made to use a fluorescent dye as a probe in a kinetic study of DNA-poly-L-lysine interaction by the stopped-flow method. It is found that 9-aminoacridine is suitable for this purpose. The results have indicated that polylysine binds to DNA at least in two steps; a slow unimolecular process (τ ～ 2 msec) being preceded by a rapid bimolecular one (τ ? 1 msec). The usefulness of this method for the kinetic study of the interaction between DNA and basic polypeptides has been emphasized. |
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