A two-locus molecular characterization of Paramecium calkinsi |
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Authors: | Przyboś Ewa Tarcz Sebastian Potekhin Alexey Rautian Maria Prajer Małgorzata |
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Institution: | 1. Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Kraków 31-016, S?awkowska 17, Poland;2. Department of Microbiology, Faculty of Biology and Soil Science, St. Petersburg State University, Saint Petersburg 199034, Universitetskaya nab. 7/9, Russia;3. Laboratory of Protozoan Karyology, Biological Institute of St. Petersburg State University, Saint Petersburg 198504, Oranienbaumskoye shosse 2, Russia |
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Abstract: | Paramecium calkinsi (Ciliophora, Protozoa) is a euryhaline species which was first identified in freshwater habitats, but subsequently several strains were also collected from brackish water. It is characterized by clockwise spiral swimming movement and the general morphology of the "bursaria type." The present paper is the first molecular characterization of P. calkinsi strains recently collected in distant regions in Russia using ITS1-5.8S- ITS2-5'LSU rDNA (1100bp) and COI (620bp) mtDNA sequenced gene fragments. For comparison, our molecular analysis includes P. bursaria, exhibiting a similar "bursaria morphotype" as well as species representing the "aurelia type," i.e., P. caudatum, P. multimicronucleatum, P. jenningsi, and P. schewiakoffi, and some species of the P. aurelia species complex (P. primaurelia, P. tetraurelia, P. sexaurelia, and P. tredecaurelia). We also use data from GenBank concerning other species in the genus Paramecium and Tetrahymena (which used as an outgroup). The division of the genus Paramecium into four subgenera (proposed by Fokin et al. 2004) is clearly presented by the trees. There is a clear separation between P. calkinsi strains collected from different regions (races). Consequently, given the molecular distances between them, it seems that these races may represent different syngens within the species. |
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Keywords: | COI mtDNA intraspecific polymorphism ITS1-5 8S-ITS2-LSU rDNA molecular phylogeny Paramecium calkinsi |
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