Masseteric Nerve Injury Increases Expression of Brain-Derived Neurotrophic Factor in Microglia Within the Rat Mesencephalic Trigeminal Tract Nucleus |
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Authors: | Hiroyuki Ichikawa Tadasu Sato Mitsuhiro Kano Toshihiko Suzuki Saburo Matsuo Hiroyasu Kanetaka Yoshinaka Shimizu |
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Institution: | (1) Division of Oral and Craniofacial Anatomy, Graduate School of Dentistry, Tohoku University, 4-1 Seiryo-machi, Sendai 980-8575, Japan;(2) Laboratory of Toxicology, Course of Veterinary Science, Graduate School of Life and Environmental Biosciences, Osaka Prefecture University, Izumisano 598-8531, Japan;(3) Graduate School of Biomedical Engineering, Tohoku University, Sendai 980-8577, Japan;(4) Division of Oral Pathology, Graduate School of Dentistry, Tohoku University, Sendai 980-8575, Japan |
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Abstract: | The distribution of brain-derived neurotrophic factor was examined in the rat mesencephalic trigeminal tract nucleus after
transection and crush of the masseteric nerve. In the intact mesencephalic trigeminal tract nucleus, brain-derived neurotrophic
factor was detected in small cells with fine processes. These cells and processes were occasionally located adjacent to tyrosine
kinase B receptor-immunoreactive sensory neurons. The transection and crush of the masseteric nerve increased expression of
brain-derived neurotrophic factor in the nucleus. The number and size of brain-derived neurotrophic factor-immunoreactive
cells and processes were dramatically elevated by the nerve injury. As a result, the density of brain-derived neurotrophic
factor-immunoreactive profiles in the mesencephalic trigeminal tract nucleus at 7 days after the injury was significantly
higher compared with the intact nucleus. Double immunofluorescence method also revealed that brain-derived neurotrophic factor-immunoreactive
cells were mostly immunoreactive for OX-42 but not glial fibrillary acidic protein. In addition, the retrograde tracing method
demonstrated that brain-derived neurotrophic factor-immunoreactive cells and processes surrounded retrogradely labeled neurons
which showed tyrosine kinase B receptor-immunoreactivity. These findings indicate that the nerve injury increases expression
of brain-derived neurotrophic factor in microglia within the mesencephalic trigeminal tract nucleus. The glial neurotrophic
factor may be associated with axonal regeneration of the injured primary proprioceptor in the trigeminal nervous system. |
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