Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes |
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Authors: | Lomonaco Sara Knabel Stephen J Dalmasso Alessandra Civera Tiziana Bottero Maria Teresa |
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Institution: | Department of Animal Pathology, Faculty of Veterinary Medicine, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy. |
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Abstract: | A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes. |
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