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Novel multiplex single nucleotide polymorphism-based method for identifying epidemic clones of Listeria monocytogenes
Authors:Lomonaco Sara  Knabel Stephen J  Dalmasso Alessandra  Civera Tiziana  Bottero Maria Teresa
Institution:Department of Animal Pathology, Faculty of Veterinary Medicine, University of Turin, Via Leonardo da Vinci 44, 10095 Grugliasco (TO), Italy.
Abstract:A novel primer extension-based, multiplex minisequencing assay targeting six highly informative single nucleotide polymorphisms (SNPs) in four virulence genes correctly identified and differentiated all four epidemic clones (ECs) of Listeria monocytogenes and 9 other strains initially misclassified as non-ECs. This assay allows rapid, accurate, and high-throughput screening for all known ECs of L. monocytogenes.
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