Cytokines mRNA in bone marrow-derived dendritic cells in asthmatic mouse |
| |
Authors: | Yu Duowei Sun Yun Wu Jianqing and Yuan Sheng |
| |
Institution: | (1) School of Life Science, Nanjing Normal University, Nanjing, 210097, China;(2) The First Affiliated Hospital of Nanjing Medical University, Najnjing, 210029, China |
| |
Abstract: | By inducing and amplifying dendritic cells (DCs) derived from the bone marrow of asthma murine in vitro, cytokines mRNA were
expressed, and the functions of DCs were investigated. Cells isolated from murine bone marrow have been cultured with rmGM-CSF
and rmIL-4, and the expression of cytokines mRNA was determined by ribonuclease protection assay combined with multi-probe
templates. Large numbers of DCs have been obtained from bone marrow, and they expressed interleukin-13 (IL-13), interleukin-9
(IL-9), and interleukin-3 (IL-3) mRNA. Moreover, the level of IL-13 mRNA and IL-9 mRNA expressed by DCs in asthmatic mice
was significantly higher than those in the control groups (P<0.05). But, the level of IL-3 mRNA showed no discrepancy between the two groups (P>0.05). DCs are very important in the forming and developing of asthma, which implies that the therapy targeted at DCs will
possibly become a new goal.
__________
Translated from Journal of Nanjing Normal University (Natural Science), 2005, 28(2): 96–100 译自: 南京师范大学学报 (自然科学版), 2005, 28(2): 96–100] |
| |
Keywords: | asthma dendritic cell (DC) cytokine interleukin-13 (IL-13) Interleukin-9 (IL-9) |
本文献已被 SpringerLink 等数据库收录! |
|