Long term maintenance of an anther-derived haploid callus line of the Asiatic hybrid lily ‘Connecticut King’ |
| |
Authors: | Han Dong-Sheng Niimi Yoshiji Nakano Masaru |
| |
Institution: | (1) Faculty of Agriculture, Niigata University, 2-8050 Ikarashi, Niigata 950-2181, Japan |
| |
Abstract: | A haploid callus line from anther cultures of the Asiatic hybrid lily ‘Connecticut King’ was maintained for a long term. The
survival and growth of the haploid calluses were affected by auxins of picloram, α-naphthaleneacetic acid (NAA) and 2,4-dichlorophenoxyacetic
acid (2,4-D) and temperatures of 25, 15 and 7 °C during culture. Picloram was more suitable for maintenance of the haploid
calluses, whereas NAA and 2,4-D led to root and shoot formation from the haploid calluses. The best temperature for maintenance
was 25 °C. About 90% of cells in calluses were maintained in haploid level during 60 weeks of subculture, and about 80% of
cells were haploid in the calluses maintained over 2 years with the MS medium containing 4 μM picloram in the dark at 25 °C.
This revised version was published online in June 2006 with corrections to the Cover Date. |
| |
Keywords: | haploid calluses Lilium picloram |
本文献已被 SpringerLink 等数据库收录! |
|