Micropropagation and acclimatization of Hedeoma multiflorum |
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Authors: | Koroch Adolfina R Juliani Héctor R Juliani Héctor R Trippi Victorio S |
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Institution: | (1) Laboratorio de Biotecnología Vegetal, Facultad de Ciencias Agropecuarias, Universidad Nacional de Córdoba, P.O. Box, 509, (5000) Córdoba, República Argentina;(2) Cátedra de Farmacognosia, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, República Argentina |
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Abstract: | A method for the micropropagation of Hedeoma multiflorum Benth from shoot tips or nodal segments was developed. Proliferating
microshoot cultures were obtained by placing shoot tips or nodal segments on half-strength Murashige and Skoog (1962) medium
supplemented with 22.2 μM BA or 22.2 μM BA plus 0.05 μM NAA. Individual shoots were excised and transferred into rooting medium
containing auxins (IBA, NAA or IAA). Rooting of shoots was better on half-strength MS medium containing 0.6 μM IAA than on
half-strength MS medium without growth regulators. Rooted plantlets were successfully acclimatized to soil. Preconditioning
at different sucrose concentrations prior to acclimatization had no effect on plant establishment, but influenced plant quality.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | aromatic plant essential oil multiple shoots |
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