Detection of protoplast-derived DNA tetraploid Lisianthus (Eustoma grandiflorum) plants by leaf and flower characteristics and by flow cytometry |
| |
| Authors: | G C Lindsay M E Hopping I E W O'Brien |
| |
| Institution: | (1) The Horticulture and Food Research Institute of New Zealand Ltd, Mt Albert Research Centre, Private Bag 92169, Auckland, New Zealand;(2) Present address: Levin Research Centre, Private Bag 4005, Levin, New Zealand |
| |
| Abstract: | Plants of lisianthus (Eustoma grandiflorum (Griesbach)Schinners=Lisianthus russellianus Hook.) were regenerated from protoplasts and grown in pots until flowering. Vegetative and floral characteristics were measured and compared with parent plants. Larger leaves and petals and longer guard cells, sepals and filaments were recorded from protoplast-derived plants suggestive of polyploidy. The nuclear DNA contents of protoplast-derived and parental plants were determined by flow cytometry. Protoplast-derived plants were confirmed as DNA tetraploid by flow cytometry with a DNA index of 1.95. Their nuclear DNA content was measured as 6.33±0.04 pg DNA per 2C nucleus compared with 3.26±0.10 pg DNA per 2C nucleus from parental plants. Polyploidisation induced during protoplast regeneration offers an alternative to that of colchicine treatment. |
| |
| Keywords: | DNA tetraploid Eustoma flow cytometry protoplast regeneration |
| 本文献已被 SpringerLink 等数据库收录！ |
