Minimal growth in vitro conservation of coffee (Coffea spp.) |
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Authors: | Anna Bertrand-Desbrunais Michel Noirot André Charrier |
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Institution: | (1) Genetic Resources and Tropical Plant Breeding Laboratory, ORSTOM, B.P. 5045, F 34032 Montpellier Cedex 01, France |
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Abstract: | We have studied the influence of low concentrations of 6-benzyladenine on growth limitation, in order to preserve coffee germplasm through a microcutting collection. Concentrations of 0 M, 1.3 M and 4.4 M were compared in four species: Coffea congensis, C. canephora, C. liberica and C. racemosa. After six months, microcutting behaviour varied between the different treatments, and a species effect was observed. The slow growing species (C. liberica and C. congensis) needed 1.3 M; the others coffee species (C. canephora and C. racemosa) exhibited moderate caulogenesis on 6-benzyladenine-free medium. Zero and low concentrations did not affect survival rates. In conclusion 1.3 M seems most appropriate for conserving all four species.Abbreviation BA
6-benzyladenine |
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Keywords: | 6-benzyladenine Coffea species growth limitation in vitro conservation shoot culture |
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