An improved method for callus proliferation and regeneration of Fuchsia hybrida |
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| Authors: | Yong Neng Chow Barbara M R Harvey Christopher Selby |
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| Institution: | (1) Department of Agricultural Botany, The Queen's University of Belfast, Newforge Lane, BT9 5PX Belfast, Northern Ireland;(2) Agricultural Botany Research Division, Department of Agriculture for Northern Ireland, Newforge Lane, BT9 5PX Belfast, Northern Ireland |
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| Abstract: | Best callus initiation was obtained when single-node explants of Fuchsia hybrida were incubated in the light on Gamborg B5 medium containing 5×10-6 M indoleacetic acid and benzylaminopurine at 5×10-7 M or 10-6 M. Healthy callus proliferation was maintained in darkness on full-strength B5 medium supplemented with 5×10-6 M IAA and 5×10-7 M BAP. Regeneration from callus was obtained in 3 to 6 weeks, using half-strength hormone-free Campbell & Durzan medium.Abbreviations BAP
6-benzylaminopurine
- 2,4-D
2,4 dichlorophenoxyacetic acid
- IAA
indoleacetic acid
- IBA
indolebutyric acid
- NAA
 -naphthaleneacetic acid
- SE
standard error |
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| Keywords: | Fuchsia hybrida callus culture regeneration |
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