Phenylpropanoid production in callus and cell suspension cultures of <Emphasis Type="Italic">Buddleja cordata</Emphasis> Kunth |
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Authors: | M E Estrada-Zúñiga F Cruz-Sosa M Rodríguez-Monroy J R Verde-Calvo E J Vernon-Carter |
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Institution: | (1) Departamento de Biotecnología, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco No. 186, Col. Vicentina, CP 09340 Mexico, DF, Mexico;(2) Departamento de Biotecnología, Centro de Desarrollo de Productos Bióticos del Instituto Politécnico Nacional, CP 62731 Yautepec, Morelos, Mexico;(3) Departamento de Ingeniería de Procesos e Hidráulica, Universidad Autónoma Metropolitana-Iztapalapa, San Rafael Atlixco No. 186, Col. Vicentina, CP 09340 Mexico, DF, Mexico |
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Abstract: | Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids),
as these metabolites are credited with therapeutic properties. Highest callus induction (76.4–84.3%) was obtained in five
treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45–9.05 μM) with Kinetin (KIN: 2.32, 4.65 μM), whereas highest root induction (79.6%) corresponded to the α-Naphthaleneacetic
acid (9.05 μM) with KIN (2.32 μM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root,
white and green callus) 66.24–86.26 mg g−1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95–3.01 mg g−1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g−1 DW and 8.12 mg g−1 DW, respectively). |
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Keywords: | Tissue culture Buddleja cordata Secondary metabolite Verbascoside Linarin Hydroxycinnamic acids |
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