Induction and characterization of in vitro corms of diploid-taro |
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Authors: | Zhou Su P He Ye K Li Shi J |
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Institution: | (1) Horticultural Department of, Nanjing Agricultural University, Nanjing, 210095;(2) National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology, Chinese Academy of Sciences, Shanghai, 200032, China |
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Abstract: | When in vitro plantlets were cultured in Murashige and Skoog liquid medium supplemented with 8–10% sucrose and 22–44 μM 6-benzylaminopurine,
all of the stem explants formed corms. 170–850 μM paclobutrazol increased corm formation, whereas 1700 μM paclobutrazol inhibited
corm development. Inclusion of 66 μM 6-benzylaminopurine in 170 μM paclobutrazol treatment resulted in smaller corms, and
bigger corms formed in the combination of 1700 μM paclobutrazol and 66 μM 6-benzylaminopurine. No corms formed in 63–630 μM
cycocel treatments. In vitro corm growth was also affected by the culture methods. Deep-layer agitated culture yielded corms of up to 2.03 g, with an
average fresh weight of 0.7 g, 40 days after induction. In thin layer cultures, corms were up to 1.87 g, with an average fresh
weight of 0.5 g. SDS-PAGE analysis of water-soluble proteins revealed changes of polypeptides with corm growth. Compared to
smaller ones, corms over 0.2 g had higher dry matter, carbohydrate and anthocyanin content. These corms had a 99–100% survival
rate upon transplanting directly to soil after storage at 4 °C for 10 months. This study indicates that the most economic
production method of diploid taro seed corm is by thin-layer liquid culture in Murashige and Skoog medium supplemented with
22–44 μM benzylaminopurine and 8–10% sucrose for 6 weeks. The formed corms can be stored at 4 °C up to 10 months and transplanted
directly into soil without acclimatization.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | BA CCC Colocasia esculenta corm PP333 sucrose |
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