Spontaneous versus colchicine-induced chromosome doubling in maize anther culture |
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Authors: | Antoine-Michard Sylvie Beckert Michel |
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Institution: | (1) INRA, Station d'Amélioration des Plantes, Domaine de Crouelle, 63039 Clermont-Fd CEDEX, France |
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Abstract: | The range of genetic variation of spontaneous chromosome doubling frequency of maize haploid plantlets derived from in vitro
anther culture was evaluated. When regeneration is obtained by direct embryo-genesis, bypassing the callus phase, it appears
that the frequency of spontaneous doubling may exceed 40 of the regenerated plantlets. This high frequency may be one consequence
of the use of doubled haploid lines derived from anther culture and spontaneous chromosome doubling. We also report an increase,
by more than 50, of the productivity of diploid fertile regenerated plantlets produced by colchicine supplemented medium during
the cold shock pretreatment of the microspores inside the anthers. Optimization of the treatments and the anther culture procedure
are discussed.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | corn diploidization genetic variability haploid plants regeneration |
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