A combination of elicitation and precursor feeding leads to increased anthocyanin synthesis in cell suspension cultures of <Emphasis Type="Italic">Vitis vinifera</Emphasis> |
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Authors: | Junge Qu Wei Zhang Xingju Yu |
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Institution: | (1) Marine Bioproducts Engineering Group, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, 116023, China;(2) Zhejiang Provincial Key Laboratory of Biopharmaceuticals Based in Ningbo, Zhejiang Pharmaceutical College, Ningbo, 315100, China;(3) CRC for Bioproducts and Department of Medical Biotechnology, School of Medicine, Flinders University, Adelaide, SA5042, Australia;(4) Department of Applied Biology, Zhejiang Pharmaceutical College, Ningbo, 315100, China |
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Abstract: | Both elicitation and precursor feeding are effective strategies for improving secondary metabolite production in plant cell
suspension cultures. In this study, cell suspension cultures of Vitis vinifera subjected to methyl jasmonate treatment resulted in a significant increase in levels of anthocyanin production. Moreover,
a combination of 5 mg/L phenylalanine and 50 mg/L methyl jasmonate promoted the highest level of anthocyanin biosynthesis,
resulting in 4.6- and 3.4-fold increases in anthocyanin content and yield, respectively, over the control. The optimum period
for elicitation of anthocyanin synthesis was 4 days following incubation in the presence of elicitors, at the beginning of
the exponential growth phase. V. vinifera cell lines of different anthocyanin-producing capabilities responded differently to elicitation and precursor feeding. Anthocyanin
production of a low-producing cell line, VV06, could be enhanced with addition of elicitors and precursor feeding. Methyl
jasmonate was the only elicitor that increased anthocyanin production of the high-producing cell line VV05, but contributed
to moderate enhancement of anthocyanin production compared with VV06. For cell line VV06, synergistic effects were observed
for all treatment combinations of methyl jasmonate along with other elicitors and precursors. In addition, 6.1- and 4.6-fold
increases in anthocyanin content and yield, respectively, were obtained in the presence of 5 mg/L phenylalanine, 50 mg/L methyl
jasmonate, and 1 mg/L dextran. However, none of these treatment combinations exhibited synergistic effects in cell line VV05. |
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