An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.) |
| |
Authors: | Guangyuan He Jinrui Zhang Kexiu Li Zhiyong Xiong Mingjie Chen Junli Chang Yuesheng Wang Guangxiao Yang Beáta Barnabás |
| |
Institution: | (1) China–UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology (HUST), 430074 Wuhan, Hubei, China;(2) Rothamsted Research (RRes), AL5 2JQ Harpenden, Hertfordshire, UK;(3) Cell Biology Department, Agricultural Research Institute of the Hungarian Academy of Sciences, 2462 Martovasar, Hungary |
| |
Abstract: | Regenerable, embryogenic haploid cell suspensions were initiated and established from type II pollen calluses of two selected Chinese maize genotypes (No 592 Y and 592.A2 LY). The induction frequency of friable, embryogenic callus (type II) was highly dependent on three factors: genotype, medium, cold pretreatment, and on their interactions. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20–50 cells. The selected cell lines were able to maintain their morphogenic ability during long-term subculture (2 years). Protoplasts were successfully isolated from subcultured, friable, embryogenic pollen calluses and cultured on N6BM and N6K media using a feeder layer, obtained from 2-day-old suspension culture. Healthy plants were regenerated from protoplast-derived calluses. |
| |
Keywords: | anther culture plant regeneration protoplast culture type II callus Zea mays |
本文献已被 SpringerLink 等数据库收录! |
|