Phloroglucinol enhances growth and rate of axillary shoot proliferation in potato shoot tip cultures in vitro |
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Authors: | Sarkar Debabrata Naik Prakash S |
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Institution: | (1) Division of Crop Improvement, Central Potato Research Institute, Shimla-, 171 001 Himachal Pradesh, India |
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Abstract: | Efficacy of phloroglucinol in promoting growth and development of in vitro-derived shoot tips was studied in six potato (Solanum tuberosum L.) genotypes. Different concentrations of phloroglucinol (0, 0.08, 0.4, 0.8, 1.2 and 1.6 mM) were tested in combination
with either 0.1 or 0.2 M sucrose in shoot tip proliferation medium based on MS (Murashige and Skoog, 1962) medium supplemented
with 5.8 μM GA3 (gibberellic acid), 1.1 μM BA (N6-benzyladenine) and 8.39 μM D-calcium pantothenate. Phloroglucinol fostered multiple shoot formation, promoted axillary shoot
proliferation in terms of shoot tip fresh weight and shoot length, and stimulated root formation on the shoot tips. There
was significant phloroglucinol × sucrose interaction for number of shoots developed per shoot tip, shoot tip fresh weight
and number of roots induced per shoot tip. The beneficial effect of phloroglucinol on shoot tip survival was conspicuous only
in genotypes that showed poor survival in the control proliferation medium. There were significant differences in response
between the two sucrose levels with regard to shoot tip fresh weight and number of roots per shoot tip. Phloroglucinol in
combination with 0.2 M sucrose induced maximum number of roots per shoot tip. Optimum shoot tip growth was fostered in medium
containing 0.8 mM phloroglucinol and 0.2 M sucrose. High frequency multiple shoot formation in this medium ensures a faster
rate of potato shoot tip multiplication within a limited time and space.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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Keywords: | 1 3 5-benzenetriol Dilospan S meristem culture micropropagation phenolic compound phloroglucinol Solanum tuberosum L 1 3 5-trihydroxybenzene |
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