Plant regeneration and genetic transformation of Lotus angustissimus |
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Authors: | E Nenz F Pupilli F Paolocci F Damiani C A Cenci S Arcioni |
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Institution: | (1) Istituto di Recerche sul Miglioramento Genetico delle Piante Foraggere del CNR, Via dell Madonna Alta, 130, 06128 Perugia, Italy;(2) Dipartimento di Biologia ed Economia Agro-Industriale, Via delle Scienze, 208, 33100 Udine, Italy |
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Abstract: | Culture conditions have been established for callus induction and growth from different explants in L. angustissimus L. Calli were obtained from hypocotyls, leaves, stems, cotyledons and roots cultured on media containing 2,4-dichlorophenoxyacetic acid or -naphthaleneacetic acid with kinetin, N6 – 2 or benzyladenine in different combinations and concentrations. Only those calli induced in presence of -naphthaleneacetic acid with benzyladenine or kinetin produced shoots. Calli induced from hypocotyl explants were the most efficient in regeneration of shoots. Transformation with an Agrobacterium rhizogenes binary vector carrying the plasmid pBI 121.1 is reported. The percentage of cotransformation was estimated by testing GUS activity in hairy roots. The integration of Ri T-DNA and the NPTII gene in transformed plants was confirmed by molecular analyses and in vitro culture of transgenic tissues in the presence of kanamycin.Abbreviations BA
benzyladenine
- 2,4-d
2,4-dichlorophenoxyacetic acid
- 1AA
indole-3-acetic acid
- NAA
-naphthaleneacetic acid
- 2iP
N6 – 2
- PA
proanthocyanidins
- NOS
nopaline synthase
- NI TII
neomycin phosphotransferase
- GUS
-glucuronidase
- CaMV
cauliflower mosaic virus |
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Keywords: | Agrobacterium rhizogenes callus shoot organogenesis tannins |
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