Maturation of black spruce somatic embryos. Part I. Effect of l-glutamine on the number and germinability of somatic embryos |
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Authors: | Slaheddine Khlin Francine M Tremblay |
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Institution: | (1) Centre de Recherche en Biologie Forestière, Faculté de Foresterie et de Géomatique, Université Laval, G1K 7P4, Québec, Canada;(2) Seconded from the Petawawa National Forestry Institute to Laval University, Canada |
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Abstract: | Different concentrations of l-glutamine and different nitrogen sources in the medium were compared during maturation of black spruce (Picea mariana (Mill.) B.S.P.) somatic embryos. l-glutamine can be used as the sole nitrogen source for the maturation of Picea mariana somatic embryos at 2 to 3 gl-1. A significantly lower number of somatic embryos was obtained on a medium prepared with only inorganic nitrogen. Compared with a medium supplement to inorganic nitrogen resulted in a twofold increase in the number of embryos for six genotypes. The nitrogen source and concentration in the maturation medium significantly affected the germination sensus stricto of somatic embryos (radicle appearance), but not their development into plantlets; at the time of epicotyl appearance, an effect of the nitrogen source was no longer found. A comparison of the development of somatic embryos into plantlets from seven genotypes showed that the genotype had more effect in terms of epicotyl appearance and in conversion rate than the nitrogen source present in the maturation medium.Abbreviations HLM-1
half-Litvays's medium with 10 M 2,4-D and 5 M BA
- i
only inorganic nitrogen in the medium
- i+1 gG
inorganic nitrogen plus 1 g l-1 glutamine in the medium
- SMM
standard maturation medium
- 2.5gG
only 2.5 g l-1 glutamine in the medium |
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Keywords: | glutamine maturation nitrogen Picea mariana somatic embryogenesis |
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