Regeneration of plants from primary leaves of cowpea |
| |
Authors: | B Muthukumar M Mariamma A Gnanam |
| |
Institution: | (1) Department of Plant Sciences, School of Biological Sciences, Madurai Kamaraj University, 625021 Madurai, India;(2) Pondicherry University, 605014 Pondicherry, India |
| |
Abstract: | Plants were regenerated from the in vitro cultured explants of primary leaves of cowpea (Vigna unguiculata L. Walp). Primary leaves, including the intact petiole, were excised from three-day-old seedlings and cultured on Gamborg's B5 basal medium containing 8×10–7 M 2,4,5-trichlorophenoxyacetic acid, 1×10–2 M L-glutamine and 1×10–4 M adenine sulfate. Callus formed at the petiole end. Prolific shoot regeneration occurred when this callus was transferred to B5 basal medium containing 5×10–6 M 6-benzyl-aminopurine (BAP). Regenerated shoots rooted in growth-regulator-free B5 basal medium and were established in soil.Abbreviations BAP
6-benzylaminopurine
- IAA
indole-3-acetic acid
- NAA
1-napthalene acetic acid
- 2,4,5-T
2,4,5-trichloro-phenoxyacetic acid |
| |
Keywords: | callus growth regulators shoot organogenesis Vigna unguiculata L |
本文献已被 SpringerLink 等数据库收录! |
|